香港六合彩开奖结果

Vol. 21 No. 2 (2022)
Original Articles

A high-fat diet and schistosomiasis mansoni cause histological changes in mice livers

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  • Carlos Eduardo da S. Filomeno,
  • Michele Costa-Silva,
  • Christiane L. Corr锚a,
  • Renata H. Neves,
  • Jos茅 Roberto Machado-Silva
DOI:

Published 2023-01-03

Keywords

  • Schistosomiasis mansoni,
  • Liver,
  • Comorbidities,
  • Lipids,
  • Stereology

How to Cite

1.
da S. Filomeno CE, Costa-Silva M, L. Corr锚a C, H. Neves R, Machado-Silva JR. A high-fat diet and schistosomiasis mansoni cause histological changes in mice livers. BJHBS [Internet]. 2023 Jan. 3 [cited 2024 Oct. 12];21(2):101-10. Available from: /bjhbs/article/view/10

Copyright (c) 2022 香港六合彩开奖结果

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Abstract

Introduction: Human and animal studies have shown that聽schistosomiasis protects against metabolic diseases such as dyslipidemia,聽atherosclerosis and diabetes. Objectives: However,聽less is known about the effects of a high-fat diet on hepatic聽architecture, formation and composition of granulomas in聽acute schistosomiasis mansoni. Methods: Male C57BL/6 mice聽fed high-fat (60% fat) chow or standard (10% fat) chow for 13聽weeks were infected with 80 Schistosoma mansoni cercariae.聽The mice were assigned into four groups: uninfected fed standard聽(SC), high-fat chow (HFC), infected fed standard (ISC) and聽high-fat chow (IHFC). Blood lipid concentrations (cholesterol,聽triglycerides, LDL, VLDL, HDL), oral glucose tolerance test,聽body mass gain, liver mass and intestinal parasite egg counting聽(oogram), cellular composition, percentage of inflammatory聽infiltrates and morphometric features (area and perimeter) of聽liver granulomas were measured. The volumetric density of聽hepatocytes, steatosis, sinusoids, necrosis and hepatic fibrosis聽were estimated by using stereology. Results: IHFC mice聽showed lower blood lipid levels, biometrics, improved glucose聽tolerance and less steatosis compared to control mice (HFC).聽The IHFC group showed larger granulomas, predominance of聽polymorphonuclear cells and rich inflammatory infiltration,聽binucleated hepatocytes and histopathological changes more聽severe than ISC. Conclusion: Although acute schistosomiasis聽reduces the effects of a high-fat diet on the host鈥檚 metabolism,聽it induces damage to liver architecture.

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