香港六合彩开奖结果

Vol. 22 No. 1 (2023)
Original Articles

Gene regulation of MMPs and TIMPs by somatostatin in human fibroblasts

pdf
  • Guilherme N. Carvalho,
  • Jean N. Santos,
  • Nara S. Araujo,
  • Patr铆cia R. Cury
Guilherme N. Carvalho
Programa de P贸s-gradua莽茫o em Odontologia e Sa煤de, Universidade Federal da Bahia, Faculdade de Odontologia, Salvador, Bahia, Brazil.
Jean N. Santos
Departamento de Patologia Oral, Universidade Federal da Bahia, Faculdade de Odontologia, Salvador, Bahia, Brazil.
Nara S. Araujo
Departamento de Periodontia, Universidade Federal da Bahia, Faculdade de Odontologia, Salvador, Bahia, Brazil.
Patr铆cia R. Cury
Departamento de Periodontia, Universidade Federal da Bahia, Faculdade de Odontologia, Salvador, Bahia, Brazil.
DOI:

Published 2023-07-17

Keywords

  • Fibroblasts,
  • Gingiva,
  • Matrix metalloproteinases,
  • Tissue inhibitor of metalloproteinases,
  • Somatostatin

How to Cite

1.
Guilherme N. Carvalho, Jean N. Santos, Nara S. Araujo, Patr铆cia R. Cury. Gene regulation of MMPs and TIMPs by somatostatin in human fibroblasts. BJHBS [Internet]. 2023 Jul. 17 [cited 2024 Oct. 12];22(1):36-44. Available from: /bjhbs/article/view/138

Copyright (c) 2023 香港六合彩开奖结果

This work is licensed under a .

Crossref
Scopus

Abstract

Introduction: Somatostatin (SST) is a commonly found neuropeptide with general inhibitory functions. The aim of this study is to evaluate the effect of somatostatin in different concentrations on the mRNA expression of MMPs and TIMPs in cultured, human, gingival fibroblasts. Methodology and resources: Human gingival fibroblasts were stimulated with 10-4, 10-9 or 10-12M somatostatin DMEM without fetal calf serum; while untreated fibroblasts served as controls. After the incubation period, the RNA was extracted and the first-strand cDNA was synthesized. Alterations in the expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-11, TIMP-1 and TIMP-2 mRNA were evaluated using real-time polymerase chain reaction (PCR). 尾-actin mRNA expression was used to normalize the data.Results: After 24 hours of treatment and at the highest concentration, SSTinduced a down-regulation of MMP-1, -2, -3 and -7 expression, and an up-regulation of MMP-11 expression; while at the lowest concentration the substance induced an up-regulation of MMP-1, -2, -3, TIMP-1 and -2 expression. Similar effects were observed after 72 hours of treatment, except for the up-regulation of TIMP-2 at the higher SST concentration, as well as an up-regulation on MMP-7 and -11 expression and a down-regulation of MMP-2 and TIMP-2 expression at the lower SST concentration. Discussion: The modulation of inflammation by SST is still unclear. The findings of this study suggest that SST can modulate the gene expression of MMPs and TIMPs by cultured fibroblasts and that its effects depend on the concentration. This may represent one of the mechanisms of inflammation suppression by SST.聽

pdf

Metrics

Metrics Loading ...

References

  1. Goetzl EJ, Sreedharan SP. Mediators of communication and adaptation in the neuroendocrine and immune systems. FASEB J. 1992 Jun;6(9):2646-52. doi: 10.1096/fasebj.6.9.1612288.
  2. Krantic S. Peptides as regulators of the immune system: emphasis on somatostatin. Peptides. 2000 Dec;21(12):1941-64. doi: 10.1016/s0196-9781(00)00347-8.
  3. Lambrecht BN. Immunologists getting nervous: neuropeptides, dendritic cells and T cell activation. Respir Res.2001;2(3):133-8. doi: 10.1186/rr49.
  4. ten Bokum AM, Hofland LJ, van Hagen PM. Somatostatin and somatostatin receptors in the immune system: a review. Eur Cytokine Netw. 2000 Jun;11(2):161-76.
  5. Pollak MN, Schally AV. Mechanisms of antineoplastic action of somatostatin analogs. Proc Soc Exp Biol Med. 1998 Feb;217(2):143-52. doi: 10.3181/00379727-217-44216.
  6. Gomes-Porras M, C谩rdenas-Salas J, 脕lvarez-Escol谩 C. Somatostatin Analogs in Clinical Practice: a Review. Int J Mol Sci. 2020 Feb 29;21(5):1682. doi: 10.3390/ijms21051682.
  7. Fioravanti A, Govoni M, La Montagna G, et al. Somatostatin 14 and joint inflammation: evidence for intraarticular efficacy of prolonged administration in rheumatoid arthritis.Drugs Exp Clin Res. 1995;21(3):97-103.
  8. Luthman J, Johansson O, Ahlstr枚m U, et al. Immunohistochemical studies of the neurochemical markers, CGRP, enkephalin, galanin, gamma-MSH, NPY, PHI, proctolin, PTH, somatostatin, SP, VIP, tyrosine hydroxylase and neurofilament in nerves and cells of the human attached gingiva. Arch Oral Biol. 1988b;33(3):149-58. doi: 10.1016/0003-9969(88)90039-8.
  9. Aguila MC, Rodriguez AM, Aguila-Mansilla HN, et al. Somatostatin antisense oligodeoxynucleotide-mediated stimulation of lymphocyte proliferation in culture. Endocrinology. 1996 May;137(5):1585-90. doi: 10.1210/endo.137.5.8612489.
  10. Kao JY, Pierzchala A, Rathinavelu S, et al. Somatostatin inhibits dendritic cell responsiveness to Helicobacter pylori. Regul Pept. 2006 Mar 15;134(1):23-9. doi: 10.1016/j.regpep.2005.11.002.
  11. Blum AM, Metwali A, Cook G, et al. Substance P modulates antigen-induced, IFN-gamma production in murine Schistosomiasis mansoni. J Immunol. 1993 Jul 1;151(1):225-33.
  12. Marabini S, Matucci-Cerinic M, Geppetti P, et al. Substance P and somatostatin levels in rheumatoid arthritis, osteoarthritis, and psoriatic arthritis synovial fluid. Ann N Y Acad Sci. 1991;632:435-6. doi: 10.1111/j.1749-6632.1991.tb33147.x.
  13. Denko CW, Malemud CJ. Age-related changes in serum growth hormone, insulin-like growth factor-1 and somatostatin in system lupus erythematosus. BMC Musculoskelet Disord. 2004b Oct 20;5(1):37. doi: 10.1186/1471-2474-5-37.
  14. Abd El-Aleem SA, Morales-Aza BM, McQueen DS, Donaldson LF. Inflammation alters somatostatin mRNA expression in sensory neurons in the rat. Eur J Neurosci. 2005 Jan;21(1):135-41. doi: 10.1111/j.1460-9568.2004.03854.x.
  15. Kinane DF, Bartold PM. Clinical relevance of the host responses of periodontitis. Periodontol 2000. 2007;43:278-93. doi: 10.1111/j.1600-0757.2006.00169.x.
  16. Nissinen L, K盲h盲ri VM. Matrix metalloproteinases in inflammation. Biochim Biophys Acta. 2014 Aug;1840(8):2571-80. doi: 10.1016/j.bbagen.2014.03.007.
  17. Woessner JF Jr. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J. 1991 May;5(8):2145-54.
  18. Bode W, Fernandez-Catalan C, Grams F, et al. Insights into MMP-TIMP interactions. Ann N Y Acad Sci. 1999 Jun 30;878:73-91. doi: 10.1111/j.1749-6632.1999.tb07675.x.
  19. Birkedal-Hansen H, Moore WG, Bodden MK, et al. Matrix metalloproteinases: a review. Crit Rev Oral Biol Med. 1993;4(2):197-250. doi: 10.1177/10454411930040020401.
  20. Cury PR, Ara煤jo VC, Canavez F, et al. Hydrocortisone affects the expression of matrix metalloproteinases (MMP-1, -2, -3, -7, and -11) and tissue inhibitor of matrix metalloproteinases (TIMP-1) in human gingival fibroblasts. J Periodontol. 2007a Jul;78(7):1309-15. doi: 10.1902/jop.2007.060225.
  21. Cury PR, de Ara煤jo VC, Canavez F, et al. The effect of epidermal growth factor on matrix metalloproteinases and tissue inhibitors of metalloproteinase gene expression in cultured human gingival fibroblasts. Arch Oral Biol. 2007b Jun;52(6):585-90. doi: 10.1016/j.archoralbio.2006.11.006.
  22. Cury PR, Canavez F, de Ara煤jo VC, et al. Substance Pregulates the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in cultured human
  23. gingival fibroblasts. J Periodontal Res. 2008 Jun;43(3):255-60. doi: 10.1111/j.1600-0765.2007.01022.x.
  24. Yamada H, Nishimura F, Naruishi K, et al. Phenytoin and cyclosporin A suppress the expression of MMP-1, TIMP-1, and cathepsin L, but not cathepsin B in cultured gingival fibroblasts. J Periodontol. 2000 Jun;71(6):955-60. doi: 10.1902/jop.2000.71.6.955.
  25. Gan L, Ye S, Chu A, et al. Identification of cathepsin B as a mediator of neuronal death induced by Abeta-activated microglial cells using a functional genomics approach. J Biol Chem. 2004 Feb 13;279(7):5565-72. doi: 10.1074/jbc.M306183200.
  26. Green PG, Basbaum AI, Levine JD. Sensory neuropeptide interactions in the production of plasma extravasation in the rat. Neuroscience. 1992 Oct;50(3):745-9. doi:10.1016/0306-4522(92)90461-a.
  27. Denko CW, Malemud CJ. The serum growth hormone to somatostatin ratio is skewed upward in rheumatoid arthritis patients. Front Biosci. 2004a May 1;9:1660-4. doi:10.2741/1354.
  28. Carrasco E, Hern谩ndez C, Miralles A, et al. Lower somatostatin expression is an early event in diabetic retinopathy and is associated with retinal neurodegeneration. Diabetes Care. 2007 Nov;30(11):2902-8. doi: 10.2337/dc07-0332.
  29. Misery L, Bourchanny D, Kanitakis J, et al. Modulation of substance P and somatostatin receptors in cutaneous lymphocytic inflammatory and tumoral infiltrates. J Eur Acad Dermatol Venereol. 2001 May;15(3):238-41. doi:10.1046/j.1468-3083.2001.00259.x.
  30. Mazzeo A, Arroba AI, Beltramo E, et al. Somatostatin protects human retinal pericytes from inflammation mediated by microglia. Exp Eye Res. 2017 Nov;164:46-54. doi:10.1016/j.exer.2013wsx7.07.011.
  31. Sliwinska-Mosson M, Marek G, Grzebieniak Z, et al. Relationship between somatostatin and interleukin-6: A cross-sectional study in patients with acute pancreatitis. Pancreatology. 2018 Dec;18(8):885-891. doi: 10.1016/j.pan.2018.09.013.
  32. Kawakami Y, Adachi T, Ono S, et al. Superiority of somatostatin analog in comparison with drugs for treating pancreatic fistula in rats. Int Surg. 2020 Mar 25. doi: 10.9738/INTSURG-D-18-00040.1.
  33. Matucci-Cerinic M, Borrelli F, Generini S, et al. Somatostatin-induced modulation of inflammation in experimental arthritis. Arthritis Rheum. 1995 Nov;38(11):1687-93. doi:10.1002/art.1780381122.
  34. Heppelmann B, Pawlak M. Inhibitory effect of somatostatin on the mechanosensitivity of articular afferents in normal and inflamed knee joints of the rat. Pain. 1997Dec;73(3):377-382. doi: 10.1016/S0304-3959(97)00124-3.
  35. Casnici C, Lattuada D, Crotta K, et al. Anti-inflammatory Effect of Somatostatin Analogue Octreotide on Rheumatoid Arthritis Synoviocytes. Inflammation. 2018 Oct;41(5):1648-1660. doi: 10.1007/s10753-018-0808-5.
  36. Schmelz M, Petersen LJ. Neurogenic inflammation in human and rodent skin. News Physiol Sci. 2001 Feb;16:33-7. doi: 10.1152/physiologyonline.2001.16.1.33.
  37. Sert S, Sakallio臒lu U, L眉tfio臒lu M, et al. Neurogenic inflammation in periimplant and periodontal disease: A case-control split-mouth study. Clin Oral Implants Res. 2019 Aug;30(8):800-807. doi: 10.1111/clr.13486.
  38. Luthman J, Dahll枚f G, Mod猫er T, et al. Immunohistochemical study of neuronal markers in human gingiva with phenytoin-induced overgrowth. Scand J Dent Res. 1988a Aug;96(4):339-46. doi: 10.1111/j.1600-0722.1988.tb01565.x.
  39. Matziari M, Dive V, Yiotakis A. Matrix metalloproteinase 11 (MMP-11; stromelysin-3) and synthetic inhibitors. Med Res Rev. 2007 Jul;27(4):528-52. doi: 10.1002/med.20066.
  40. Emingil G, Tervahartiala T, M茫ntyl茫 P, et al. Gingival crevicular fluid matrix metalloproteinase (MMP)-7, extracellular MMP inducer, and tissue inhibitor of MMP-1 levels in periodontal disease. J Periodontol. 2006 Dec;77(12):2040-50. doi: 10.1902/jop.2006.060144.