@article{Guilherme N. Carvalho_Jean N. Santos_Nara S. Araujo_Patrícia R. Cury_2023, place={Rio de Janeiro - RJ, Brazil}, title={Gene regulation of MMPs and TIMPs by somatostatin in human fibroblasts}, volume={22}, url={/bjhbs/article/view/138}, DOI={10.12957/bjhbs.2023.77259}, abstractNote={<p>Introduction: Somatostatin (SST) is a commonly found neuropeptide with general inhibitory functions. The aim of this study is to evaluate the effect of somatostatin in different concentrations on the mRNA expression of MMPs and TIMPs in cultured, human, gingival fibroblasts. Methodology and resources: Human gingival fibroblasts were stimulated with 10-4, 10-9 or 10-12M somatostatin DMEM without fetal calf serum; while untreated fibroblasts served as controls. After the incubation period, the RNA was extracted and the first-strand cDNA was synthesized. Alterations in the expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-11, TIMP-1 and TIMP-2 mRNA were evaluated using real-time polymerase chain reaction (PCR). β-actin mRNA expression was used to normalize the data.Results: After 24 hours of treatment and at the highest concentration, SSTinduced a down-regulation of MMP-1, -2, -3 and -7 expression, and an up-regulation of MMP-11 expression; while at the lowest concentration the substance induced an up-regulation of MMP-1, -2, -3, TIMP-1 and -2 expression. Similar effects were observed after 72 hours of treatment, except for the up-regulation of TIMP-2 at the higher SST concentration, as well as an up-regulation on MMP-7 and -11 expression and a down-regulation of MMP-2 and TIMP-2 expression at the lower SST concentration. Discussion: The modulation of inflammation by SST is still unclear. The findings of this study suggest that SST can modulate the gene expression of MMPs and TIMPs by cultured fibroblasts and that its effects depend on the concentration. This may represent one of the mechanisms of inflammation suppression by SST. </p>}, number={1}, journal={Ïã¸ÛÁùºÏ²Ê¿ª½±½á¹û}, author={Guilherme N. Carvalho and Jean N. Santos and Nara S. Araujo and Patrícia R. Cury}, year={2023}, month={Jul.}, pages={36–44} }